Chinese Journal of Oncology 2012;34(5):348-351
doi:10. 3760/cma.j.issn.0253-3766.2012.05.006
Expression of hypoxia inducible factor-1α and insulin in pancreatic cancer and their correlation.
Tong-ling WANG 1 ; Tao LIU ; Shan-miao GOU ; Li WANG ; Wei ZHOU ; Chun-you WANG
Affiliations
Country
China
Language
Chinese
MeSH
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Abstract
OBJECTIVETo explore the correlation between the expressions of hypoxia inducible factor-1α (HIF-1α) and insulin in pancreatic cancer.
METHODSHIF-1α and insulin expression was detected by immunohistochemistry in the center and the edge of pancreatic adenocarcinoma specimens of 65 cases. Western blot was used to detect HIF-1α expression and insulin level in the center and the edge of pancreatic adenocarcinoma specimens of 28 cases. The relationship between HIF-1α expression and insulin level in the pancreatic cancer was analyzed.
RESULTSThe results of immunohistochemistry and Western blot showed that HIF-1α protein expression was high in both the center and the edge of pancreatic cancers (P > 0.05), and insulin level was significantly higher at the edge of specimen than that in the center (P < 0.05). HIF-1α protein and insulin levels were positively correlated at the edge of cancer tissue (r = 0.374, P < 0.05), but no significant correlation between them in the center of cancerous tissue (r = -0.145, P > 0.05).
CONCLUSIONInsulin may promote the local invasion and metastasis of pancreatic cancer by up-regulating HIF-1α.
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