Chinese Journal of Surgery 2007;45(4):258-261

Effects of LRIG1 gene on the invasion of bladder cancer in vitro.

Zhang-Qun YE 1 ; Ze-Jun YAN ; Wei-Min YANG ; Dong-Sheng GUO

Affiliations

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Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo observe the change of invasion ability of the bladder cancer cell line BIU87 after transfected with LRIG1 gene.

METHODSPlasmid pLRIG1-GFP was transfected into bladder cancer cells (BIU87) by Lipofectamine 2000, and cells that could express LRIG1 stably was screened out by G418. The alteration of cellular invasion property of BIU87, BIU87-neo and BIU87-LRIG1 cells was evaluated by Boyden chamber. The expression of mRNA and protein of LRIG1 and EGFR was detected by RT-PCR and Western-blot.

RESULTSCompared with BIU87 and BIU87-neo cells, after BIU87 cell line was transfected with plasmid pLRIG1-GFP, the invasion ability decreased significantly, the expression of mRNA and protein of LRIG1 was obviously up-regulated and that of EGFR was down-regulated (P < 0.01).

CONCLUSIONLRIG1 gene can significantly inhibit the invasion ability of BIU87 cell line.