Biomedical and Environmental Sciences 2018;31(1):48-56

doi:10.3967/bes2018.005

Rapid Identification and Subtyping of Enterobacter cloacae Clinical Isolates Using Peptide Mass Fingerprinting.

Yi Qian WANG 1 ; Di XIAO 1 ; Juan LI 1 ; Hui Fang ZHANG 1 ; Bao Qing FU 2 ; Xiao Ling WANG 3 ; Xiao Man AI 4 ; Yan Wen XIONG 1 ; Jian Zhong ZHANG 1 ; Chang Yun YE 1

Affiliations

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Keywords

E.cloacae; Identification; Peptide mass fingerprinting

Country

China

Language

English

Abstract

OBJECTIVETo establish a domestic database of Enterobacteria cloacae (E. cloacae), and improve the identification efficiency using peptide mass fingerprinting.

METHODSPeptide mass fingerprinting was used for the identification and subtyping of E. cloacae. Eighty-seven strains, identified based on hsp60 genotyping, were used to construct and evaluate a new reference database.

RESULTSCompared with the original reference database, the identification efficiency and accuracy of the new reference database was greatly improved at the species level. The first super reference database for E. cloacae identification was also constructed and evaluated. Based on the super reference database and the main spectra projection dendrogram, E. cloacae strains were divided into two clades.

CONCLUSIONPeptide mass fingerprinting is a powerful method to identify and subtype E. cloacae, and the use of this method will allow us to obtain more information to understand the heterogeneous organism E. cloacae.