Chinese Journal of Applied Physiology 2008;24(3):291-295

The method of long-term potentiation recording in hippocampus in anaesthetized mice in vivo.

Yan HUANG 1 ; Sheng YANG ; Wen-Xia ZHOU ; Yong-Xiang ZHANG

Affiliations

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Country

China

Language

Chinese

Abstract

AIMTo establish the method of long-term potentiation (LTP) recording in hippocampus in anaesthetized mice in vivo.

METHODSMouse was anaesthetized and then placed in the stereotaxic apparatus. The recording electrode was located at the cell body layer of dentate granule cells and the stimulating electrode at the perforant path according to stereotaxic parameters. Then the LTP was evoked and recorded.

RESULTSAfter optimizing of experimental factor, the LTP in PP-DG path in anaesthetized Balb/c mice was successfully recorded. The changes of synaptic plasticity were also observed in SAMP8 and SAMRI by using the optimized method. The results were coincident with the behavioral tests and LTP in hippocampal slices that we reported before.

CONCLUSIONThe method of LTP recording in vivo in hippocampus in anaesthetized mice was successfully established and could be used to evaluate the synaptic plasticity in vivo.