China Journal of Chinese Materia Medica 2007;32(20):2161-2165

Experimental studies of effects of Wujiwan extracts in different compatibilities on motility of isolated colon in guinea pig.

Ya-Jie WANG 1 ; Yu DONG ; Xiao-Xin ZHU

Affiliations

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Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo investigate the effects of Wujiwan extracts in two different compatibility proportions (named the No. 1 and No. 2 respectively) on motility of isolated colon in guinea pig, and compare the therapeutic effects with each other.

METHODTo observe the function of the No. 1 and No. 2 with different concentrations on colon contraction induced by acetylcholine (Ach) and inhibition induced by adrenalin (Ad) respectively, and calculate the EC, with the method of Bliss.

RESULTThe No. 1 and No. 2 both could significantly inhibit the contraction of colon, and in 15 minutes, there was significant time-dependence and concentration-dependence, and EC50 of the No. 1 was lower than that of No. 2. However, but when the colonic was inhibited by Ad, at the time of 9 min and 15 min, the No. 1 at the level of 300 mg x L(-1), and at the time of 15 min, the No. 2 at the level of 30 mg x L(-1), could both excite the colon significantly (P < 0. 05).

CONCLUSIONOur experiments indicate that the No. 1 and No. 2 both can significantly inhibit the colon contraction in guinea pig, and the No. 1 is superior to the No. 2 to some extent. And at the same time, we also can conclude that the No. 1 and No. 2 have exciting effects on inhibitory colon. Meanwhile, the No. 1 and No. 2 each have its own advantages based on the common therapeutic effects.