Chinese Journal of Plastic Surgery 2002;18(6):338-340

Influence of endothelial cells on the proliferation of scar-derived fibroblast in hypertropic scar tissue.

Linxi ZHANG 1 ; Shuzhong GUO ; Wensen XIA ; Li YANG ; Zhen WANG

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo investigate the influence of the endothelial cells on the scar-derived fibroblasts in the hypertropic scar tissue.

METHODSThe endothelial cell from the human umbilical vein was cultured and its supernatant was then collected. Thereafter, it was mixed with the pre-cultured scar-derived fibroblasts from the hypertropic scar tissue. The fibroblasts were analyzed with a flow cytometer in a MTT-assay method to evaluate the cell proliferation and its division cycle.

RESULTSThe endothelial cells were significantly increasing the scar-derived fibroblast proliferation (P < 0.05), compared with the control. The proportion of the fibroblasts in the S-stage of cell cycle was also quite high.

CONCLUSIONThe endothelial cells may secrete some active products, which may enhance the proliferation of the scar-derived fibroblasts and stimulate the fibroblasts into the S-stage of cell generation cycle.