China Journal of Chinese Materia Medica 2012;37(1):75-78

Simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C by HPLC.

Ming KONG 1 ; Zhanli CHEN ; Zhiqi YIN ; Jian ZHANG

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo develop a high-performance liquid chromatography (HPLC) method for simultaneous determination of hypocrellin A, hypocrellin B, and hypocrellin C.

METHODThe separation was carried out on a Kromasil C18 (4.6 mm x 250 mm, 5 micrm) colum eluted with in mobile phases of water containing 0.5% glacial acetic acid and acetonitrile. The column temperature was 35 degrees C, and the flow rate was 1.0 mL x min(-1). The detection wavelength was set at 265 nm.

RESULTThe three compounds were well separated. Calibration curves of hypocrellin A, hypocrellin B, and hypocrellin C showed good linear relationship RSD > 2.0%. The average recoveries of the hypocrellin A, hypocrellin B, and hypocrellin C were 101.8%, 102.3%, 100.0%, respectively.

CONCLUSIONThe developed method is simple, accurate, and repeatable, and can be readily used as valid tool for the quality control of Hypocrella bambusae.