Chinese Journal of Applied Physiology 2005;21(3):353-355

Determination of homocysteine level in plasma by high performance liquids chromatography with fluorescence detection.

Xu DA 1 ; Ling-Jia QIAN

Affiliations

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Country

China

Language

Chinese

Abstract

AIMTo establish a method for detection of plasma total homocysteine with HPLC.

METHODSThe chromatography analysis was carried out using a Symmetry Shield RP18. The mobile phase was sodium acetate (0.08 mol/L) and methanol (1%) and we utilized a HPLC system with fluorescence detection of plasma homocysteine derivatized from reaction with 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F).

RESULTSThe average recoveries were 95.8 - 100.8% and the relative standard deviations were 1.2-2.0%.

CONCLUSIONThe results showed it to be a rapid and accurate method for the determination of homocysteine level in plasma.