Chinese Journal of Applied Physiology 2005;21(3):356-358

A cell model of ischemia/reperfusion injury on skeletal muscle.

Hong-Jie LI 1 ; Guang-Ling ZHANG ; Lian-Yuan ZHANG ; Shu-Yun DONG ; Xiu-Li MEN

Affiliations

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Country

China

Language

Chinese

Abstract

AIMTo establish a model of ischemia/reperfusion injury on L-6TG cell.

METHODSCultured L-6TG cells were divided into 2 groups: control group (C), ischemia/reperfusion group (I/R), LDH in culture fluid, SOD, XOD, free calcium in L-6TG cell and mitochondria respiration were evaluated in each group, the micromorphologic changes were observed with microscope.

RESULTSCompared with control group, after L-6TG cell suffered ischemia 4 hours and reperfusion 4 hours, LDH in culture fluid, XOD, free calcium in L-6TG cell all increased significantly, while SOD in L-6TG cell and mitochondrial respiration decreased, structural damage to L-6TG cell was severe.

CONCLUSIONUsing mimicking ischemic solution and mimicking reperfusion solution can successfully establish a model of ischemia/reperfusion injury on L-6TG cell.