Chinese Journal of Virology 2011;27(5):409-415

Construction of the HSV-1 strain HF amplicon and study on its unversal function between different HSV serotypes.

Bo SONG 1 ; Xin-Jing LIU ; Zhi-Qiang HAN ; Lu ZHAO ; Qing-Zhi WANG ; Jia-Meng LU ; Yu-Ming XU

Affiliations

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Country

China

Language

Chinese

Abstract

The study aimed to construct the amplicon vector of HSV-1 strain HF and explore its universal package function between different serotypes of HSV. OriS and pac elements were obtained by enzyme digestion from the Plasmid BAC-HSV-1 strain HF and sequenced. With red fluorescence (DsRed) as a reporter gene, the amplicon vector of HSV-1 strain HF was constructed based on pSilencer2.0-U6. The amplicon vector was transfected into Vero cells by lipofectamine 2000, then packaged by HSV-1 strain HF and HSV-2 strain HG52 as helper virus separately. The supernatant was collected after cytopathic effect. Red fluorescence was observed in Vero cells reinfected by the supernatant. In this study,the amplicon vector of HSV-1 strain HF was successfully constructed and it could be packaged by HSV-1 strain HF and HSV-2 strainHG52.