Chinese Journal of Medical Genetics 2007;24(2):128-130

Cloning and characterization of down-regulated genes in neural tube defects of golden hamster induced by hyperthermia.

Qing YANG 1 ; Ying-mao GAO ; Shao-ling LI

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo clone down-regulated genes in neural tube defects of golden hamster induced by hyperthermia and to explore the molecular mechanism.

METHODSA reversed subtractive cDNA library was constructed using suppression subtractive hybridization. Clones with inserts were selected by combination of alpha complementary phenomenon and colony PCR. Then sequence and homology analysis of the inserts were made. And mRNA expression conditions were confirmed by Northern blot.

RESULTSThe reversed subtractive cDNA library was successfully constructed. Fifteen recombinant clones were sequenced and recognized homologous to known genes. The results of Northern blot showed that all these genes were down-regulated in defected neural tubes induced by hyperthermia compared to normal developed neural tubes.

CONCLUSIONSome important genes are identified which might be involved in neural tube defects induced by hyperthermia.