Asian Journal of Andrology 2002;4(4):265-268

PCR analysis of Yq microdeletions in infertile males, a study from South India.

S Ramesh BABU 1 ; M SWARNA ; P PADMAVATHI ; P P REDDY

Affiliations

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Country

China

Language

English

Abstract

AIMTo estimate the frequency of microdeletions in the long arm of Y-chromosome of 20 infertile males from South India.

METHODSPolymerase chain reaction (PCR) amplification using Y-specific STS of azoospermia factor (AZF) regions i.e., SY 84 for AZFa, SY 127 for AZFb and SY 254 for AZFc.

RESULTSOf the 20 infertile subjects 3 (15 %), one azoospermic and two oligozoospermic, showed microdeletions in the AZF region of Y-chromosome.

CONCLUSIONThe frequency of deletions involving AZF region of the Y-chromosome is 15 % in azoospermic and severely oligozoospermic infertile men. PCR amplification of AZF locus is useful for the diagnosis of microdeletions in the Y-chromosome.