Chinese Journal of Experimental and Clinical Virology 2002;16(2):160-161

Detection of the HBV DNA with Sybr green 1 realtime polymerase chain reaction.

Yan WANG 1 ; Xiaoyuan XU ; Wei HE ; Zhihong LIU ; Weibo GONG ; Qinhuan WANG

Affiliations

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Country

China

Language

Chinese

Abstract

BACKGROUNDTo search a novel sensitive, specific and lower cost method applicable for quantitative analysis of the hepatitis B virus DNA extensively.

METHODSQuantitative analysis of the DNA from 100 sera by real-time PCR with Sybr green 1. The results of Sybr's assay were compared with the results obtained with Taqman's fluorescent quantitative assay.

RESULTSTaqman real-time PCR could help evaluate the level of virus reliably. The results of Sybr's assay were in agreement with the Taqman's assay, but detection rate was lower.

CONCLUSIONSSybr green 1 real-time PCR appeared to be convenient and cheap, but detection rate was lower.