China Journal of Chinese Materia Medica 2016;41(23):4436-4441

doi:10.4268/cjcmm20162323

Analysis on pharmacokinetics-pharmacodynamics correlation of effective ingredients of Qing'e wan.

Ling LIU 1 ; Ze-Bin WENG 2 ; Heng WANG 1 ; Xing-Yu ZHU 1 ; Zhi-Peng CHEN 1 ; Li WU 1 ; Wei-Dong LI 1

Affiliations

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Keywords

Qing′e wan; geniposidic acid; isopsoralen; pharmacodynamics; pharmacokinetics; psoralen

Country

China

Language

Chinese

Abstract

To study the pharmacokinetics of three active ingredients in Qing'e wan, namely geniposidic acid, psoralen and isopsoralen, in rats, in order to investigate their correlation in the anti-osteoporotic effect. The rats were taken blood from their eye sockets at different time points after being orally administered with raw and salt-processed Qing'e wan. Geniposidic acid, psoralen and isopsoralen in rats plasma were determined by means of UHPLC-MS/MS to draw the concentration-time curve. The proliferation rate of osteoblasts was taken as the pharmacodynamic index, and determined by MTT method to draw effect-time curve. In comparison between the effect-time curve and the concentration-time curve, the blood concentrations of geniposidic acid and psoralen were close to the peak when the cell proliferation rate reached its peak, indicating a good correlation between them. The peak blood concentration of isopsoralen was slightly lagging behind the peak of efficacy. According to the correlation analysis after fitting the effect-time curve and the concentration-time curve, salt-processed Qing'e wan had a better correlation than the raw one. The above experimental results showed that the effect-time curve and the concentration-time curve of geniposidic acid and psoralen had a good correlation, and the correlation of salt-processed Qing'e wan was better than the raw one.