Biomedical and Environmental Sciences 2014;27(11):898-901

doi:10.3967/bes2014.126

Rapid Identification of Bacterial Species Associated with Bronchiectasis via Metagenomic Approach.

Dong Hong YANG 1 ; Yuan Yuan ZHANG 2 ; Peng Cheng DU 2 ; Li XU 1 ; Hai Yin WANG 2 ; Na HAN 2 ; Chen CHEN 2 ; Zhan Cheng GAO 1

Affiliations

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Country

China

Language

English

MeSH

Abstract

Bronchiectasis is a chronic lung disorder and a number of bacterial pathogens are involved. However, 30%-40% of sputum and purulent samples in good quality failed to grow any pathogenic bacteria, making it difficult to confirm the pathogen. In this study, we collected bronchoalveolar lavage fluid from a bronchiectasis patient undergoing acute exacerbation, and sent for 16S rDNA pyrosequencing by a 454 GS Junior machine. Metagenomic analysis showed the composition of bacterial community in sample was complex. More than a half of reads (51.3%) were from Pseudomonas aeruginosa. This result was corresponding with the culture result but came out 2 d earlier, which is meaningful for early diagnosis and treatment. The detection with 16S rDNA pyrosequencing technology is more sensitive and rapid than routine culture, and can detect the co-infection or symbiosis in airway, giving us a novel and convenient approach to perform rapid diagnosis.