Chinese Journal of Experimental and Clinical Virology 2008;22(2):110-112

Genome analysis of a newly isolated enterovirus.

Jun HOU 1 ; Yan HU ; Hong-hui SHENG ; Bing-ke BAI ; Zhi-jie WANG ; Pan-yong MAO

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo demonstrate molecular characterization of a newly isolated enterovirus.

METHODSVirus were isolated from patient with unknown-causing disease and tested by reverse transcription-polymerase chain reaction (RT-PCR) and 5'3'RACE (rapid amplification of cDNA ends, RACE), in an attempt to obtain the sequence of this newly isolated enterovirus.

RESULTSSequence analysis showed that this newly isolated enterovirus shared 83%-94% nucleotide identity and 91%-100% amino acid identity with enterovirus 89. Phylogenetic analysis indicated that it was probably a new subtype of enterovirus 89.

CONCLUSIONThis newly isolated enterovirus in the stool specimen from patient has the same serotype with enterovirus 89, but it was probably a new subtype of enterovirus 89.