Biomedical and Environmental Sciences 2014;27(2):134-137

doi:10.3967/bes2014.029

Lateral flow immunoassay for quantitative detection of ractopamine in swine urine.

Mei Ling REN 1 ; Xue Lan CHEN 2 ; Chao Hui LI 1 ; Bo XU 3 ; Wen Juan LIU 3 ; Heng Yi XU 1 ; Yong Hua XIONG 1

Affiliations

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Country

China

Language

English

Abstract

A strip reader based lateral flow immunoassay (LFIA) was established for the rapid and quantitative detection of ractopamine (RAC) in swine urine. The ratio of the optical densities (ODs) of the test line (AT) to that of the control line (AC) was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration (IC50) of 0.59 ± 0.06 ng/mL. The limit of detection (LOD) of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.