Chinese Journal of Medical Genetics 2002;19(5):386-388

Study on point mutations in mitochondrial DNA control region for replication DLP(6) of cultured dermal fibroblast with 8-MOP/UVA treatment.

Zhongrong LIU 1 ; Rongqing LIU ; Guowei ZHANG ; Fei HAO ; Heng YAN

Affiliations

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Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo investigate the relationships between skin photoaging and point mutations in mitochondrial DNA (mtDNA) control region for replication of dermal fibroblast.

METHODSCultured dermal fibroblasts were treated by 8-methoxypsora len /ultraviolet A (8-MOP/UVA). mtDNA was extracted by one-step-method and th e PCR products of D-loop and adjacent transcription promoter (DLP(6)) fragment of mtDNA control region for replication were detected by polymerase chain reaction-single strain conformation polymorphism and direct sequencing.

RESULTSAfter treated by 8-MOP/UVA, point mutations of 414 T-->G of DLP(6) fragment of mtDNA control region for replication largely accumulated.

CONCLUSIONAccumulation of point mutations of DLP(6) fragment of mtDNA control region for replication may be closely associated with skin photoaging.