Chinese Journal of Pathology 2012;41(5):314-319

doi:10.3760/cma.j.issn.0529-5807.2012.05.007

Correlation of CpG methylation status of Runx3 with pathogenesis of gastric carcinoma.

Guo-hua TANG 1 2 ; Shao-wei SUN ; Xiu-sheng HE

Affiliations

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Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo investigate the role of Runx3 gene CpG island methylation in the development of human gastric carcinoma.

METHODSA total of 150 tumor specimens from patients with gastric carcinoma and 50 normal tissue specimens were selected. Methylation specific PCR (MSP) and pyrosequencing (PS) were used to detect the methylation status of Runx3 gene promoter.

RESULTSCompared to normal tissue samples, a significant increase of CpG island methylation status of Runx3 gene was observed in gastric carcinomas (MSP: 67.3% vs. 40.0%, P = 0.002; PS: 76.0% vs. 30.0%, P < 0.01). Runx3 gene methylation was only related to tumor size (P < 0.05) based on MSP analysis. PS test however showed that the extent of methylation of Runx3 gene was related to the tumor size (P = 0.004), Lauren's classification (P = 0.043), depth of invasion (P < 0.01), lymph node metastasis (P = 0.021) and TNM staging (P = 0.045).

CONCLUSIONSMethylation status of Runx3 gene detectable by PS is closely correlated with clinicopathological parameters of gastric carcinoma, including tumor size, Lauren's classification, depth of invasion, lymph node metastasis and TNM staging. PS is more sensitive than MSP in the detection of Runx3 gene methylation, which may serve as an important marker for early diagnosis and treatment of gastric carcinoma.