Immunology plays an important role in prevention, discovery, diagnosis and treatment. There are 4 major immunological techniques including ELISA (enzyme linked immuno-sorbent assay), ICA (immuno-chromatography assay), GLORIA (gold labeled optical read rapid immunoassay) and MCIA (membrane capture immunoassay). These techniques with the simple test devices such as test paper, test stick, test cassette provided the rapid results. The immunological techniques have been applying in emergency tests (myocardial infection, embolism, thrombosis, and occlusion), infection viral infection, allergy, cancer and drug abuse