Journal of Southern Medical University 2013;33(11):1628-1631

Influence of electroporation on immunogenicity of the DNA vaccine pVAX-tG250FcGB.

Yi XIAO 1 ; Kun GAO ; Yong YANG ; Jinqi YAN ; Liang ZHANG ; Yu WANG ; Yuanji XU ; Renli TIAN ; Zhiyan DU ; Jiyun YU

Affiliations

+expand

Country

China

Language

Chinese

MeSH

Abstract

OBJECTIVETo investigate the influence of electroporation on the immunogenicity of the DNA vaccine pVAX- tG250FcGB.

METHODSThe DNA vaccine pVAX-tG250FcGB was constructed by inserting the coding gene of tG250 fusion genes into the expression vector pVAX. The DNA vaccine was delivered in BALB/c mouse by electroporation or intramuscular injection, and the induced antigen specific immune responses were compared.

RESULTSThe vaccine delivered by electroporation and intramuscular injection both induced immune responses in BALB/c mouse, but electroporation produced an obviously stronger effect than intramuscular injection.

CONCLUSIONElectroporation-mediated DNA vaccine delivery can produce strong immune response in mice and is an effective means for studying the immunogenic effect of DNA vaccine pVAX-tG250FcGB.