Journal of Zhejiang University. Medical sciences 2003;32(5):390-392

Activation of transcription factors induced by low concentration of N-methyl-N'-nitro-N-nitrosoguanidine.

Gu-liang WANG 1 ; Zheng WANG ; Jun YANG ; Ying-nian YU

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo investigate the effect of MNNG on some of the transcription factors such as NF- kappaB, CREB, AP-1 and c-Myc.

METHODSThe activities of these transcription factors were measured by transient transfection assay of SEAP vectors.

RESULTThe expressions of AP-1, CREB and NF- kappaB driven reporter genes were elevated for about 1.3, 1.4 and 1.3 times in MNNG-treated cells, respectively, as compared to untreated controls. However, the exposure of MNNG had no effect on the activity of c-Myc.

CONCLUSIONThe activation of certain transcription factors might be involved in the process of untargeted mutation induced by low concentration of MNNG treatment.