Journal of Zhejiang University. Medical sciences 2003;32(5):412-414

Construction of a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.

Ping-yong YI 1 ; Hai YU ; Wen-xue MA ; Wen-jun WU ; Huai JIANG

Affiliations

+expand

Country

China

Language

Chinese

Abstract

OBJECTIVETo construct a chimeric SEA-hPLAP-1 cDNA with gene splicing by overlap extension.

METHODSThe SEA gene and a DNA fragment encoding the signal for GPI-anchor attachment of hPLAP -1 were amplified by PCR. The two amplified gene sequence was annealed to form a chimeric GPI- anchored SEA molecule with gene splicing by overlap extension. The resulting chimera was cloned in pGEM-T vector and verified by sequencing analysis.

RESULTA chimeric SEA-hPLAP-1 cDNA was successfully constructed with gene splicing by overlap extension.

CONCLUSIONGene splicing by overlap extension is a successful specific PCR technique for gene recombination.