Journal of Experimental Hematology 2007;15(3):458-461

Effects of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of HL-60 cells.

Shao-Qian CHEN 1 ; Ying DU ; Yu-Min HUANG ; Xin WANG ; Qiao-Li GU ; Zi-Ming DONG

Affiliations

+expand

Country

China

Language

Chinese

Abstract

This study was aimed to investigate the effect of chemokine-like factor superfamily 8 (CKLFSF8) on proliferation and expression of epidermal growth factor receptor (EGFR) of HL-60 cells. Expression of CKLFSF8 mRNA on HL-60 cell line was assayed by RT-PCR; the target gene was transfected into the cells by lipid vector, cell proliferation was determined by MTT assay, while expression of EGFR in HL-60 was determined by immunocytochemical technique. The results indicated that expression of CKLFSF8 existed in HL-60 cells. After transfection, cell proliferation was inhibited (P < 0.05) and the expression of EGFR in HL-60 cells was also discovered to be inhibited (P < 0.05). It is concluded that the proliferation and expression of EGFR in HL-60 cells can be inhibited by transfection of CKLFSF8. The novel chemokine may provide a new approach in the treatment of leukemia.