China Journal of Chinese Materia Medica 2016;41(6):1016-1020

doi:10.4268/cjcmm20160607

Mitogen-activated protein kinase genes of Artemisia annua and their expression analysis under Cadmium stress.

Liang-Yun ZHOU 1 ; Chao-Geng LV 1 ; Chuan-Zhi KANG 1 ; Sheng WANG 1 ; Jin-Fu TANG 1 ; Li-Ping KANG 1 ; Lan-Ping GUO 1

Affiliations

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Keywords

Artemisia annua; Cadmium stress; MAPK gene; expression analysis

Country

China

Language

Chinese

Abstract

In order to study Artemisia annua under cadmium stress, whether there are corresponding MAPK genes involved in transduction of the cadmium signal. 17 AaMAPK genes, named AaMAPK1-AaMAPK17 repectively, were finally obtained by using Trinity method for de novo assembly of transcripts from SRA database and BLAST search against AtMAPK genes and determing conserved domain using a series of bioinformatics tools. There exist 16 MAPK genes contained T[D/E]Y conserved domains among the obtained genes. The expressions of these genes were analyzed by Real-time PCR under cadmium stress. The results showed that the expressions level of AaMAPK3 and AaMAPK10 were down-regulated and MAPK7, MAPK9 and MAPK12 were up-regulated. These indicated that there exist corresponding MAPK genes involved in transduction of the cadmium signal.