Acta Academiae Medicinae Sinicae 2007;29(4):506-509

Reconstruction and transplantation of the composite skin comprising epithelial growth factor gene-transfected keratinocytes.

Shi-chu XIAO 1 ; Shi-hui ZHU ; Zhao-fan XIA ; Guang-qing WANG ; Yong-sheng WANG ; Zhi-guo LIU

Affiliations

+expand

Country

China

Language

Chinese

Abstract

OBJECTIVETo investigate the epithelial growth factor (EGF) expression of EGF gene-transfected keratinocytes and its effect on cell proliferation after grafting.

METHODSNewborn Balb/c mouse keratinocytes and gene transfected keratinocytes were seeded on the surface of acellular dermal matrix and cocultured in different ratios as follows: 1:1, 1:3, or 1:5 1 week after culture. The composite skin was grafted onto the full-thickness wound in Balb/c mouse. Specimen was harvested at interval after grafting and underwent the immunohistochemistry staining for EGF and proliferating cell nuclear antigen (PCNA).

RESULTSImmunohistochemical staining showed EGF was expressed in the newly generated epidermis 1-2 week after grafting of the composite skin comprising Balb/c mouse keratinocytes and gene-transfected keratinocytes (at the ratio of 1:5). One week after surgery, Anti-PCNA positive basal cells were more than that in composite skin containing Balb/c mouse keratinocytes alone (P<0.01).

CONCLUSIONThe gene-transfected keratinocytes expresses EGF and promotes the proliferation of keratinocytes in the early stage after transplantation.