Chinese Journal of Hematology 2005;26(3):167-169

Study on the direct MAIPA technique in the differential diagnosis of immune and non-immune thrombocytopenia.

Ping QIN 1 ; Ming HOU ; Jian-zhi SUN ; Lu LU ; Yan SHI ; Yuan-yuan ZHU ; Li-zhen LI ; Mao-hong ZHANG

Affiliations

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Country

China

Language

Chinese

Abstract

OBJECTIVETo evaluate the clinical usefulness of direct monoclonal antibody immobilization of platelet antigen (MAIPA) technique in the differential diagnosis of immune and non-immune thrombocytopenia.

METHODSPlatelet-bound autoantibodies in thrombocytopenic patients (immune and non-immune) were measured by direct MAIPA. Monoclonal antibodies against GP II b/III a, GPIb and GP I a/II a were used.

RESULTSThe positive rates of platelet-bound GP-specific autoantibodies between immune (76.4%) and non-immune thrombocytopenia (3.6%) were significantly different (P < 0.05). The direct MAIPA had a sensitivity of 76.4%, a specificity of 96.4%, and a positive predictive value of 97.1% for the diagnosis of immune thrombocytopenia. There was a significant inverse correlation between platelet-bound GP II b/III a specific autoantibody levels and platelet counts (r = -0.338, P < 0.05).

CONCLUSIONThe direct MAIPA technique can be used to differentiate immune from non-immune thrombocytopenias.