The Journal of the Korean Academy of Periodontology  2005;35(4):1097-1108

doi:10.5051/jkape.2005.35.4.1097

Osteoblastic differentiation of adult stem cells by Biphasic Calcium Phosphate.

Myoung Ku LEE 1 ; Sung Woo LIM ; Sung Hee PI ; Yun Sang KIM ; Hyung Shik SHIN ; Hyoung Keun YOU

Affiliations

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Keywords

Osteoblastic differentiation; mesenchymal stem cells; biphasic calcium phosphate(MBCP)

Country

Republic of Korea

Language

Korean

Abstract

The present study was to determine the influence of micro-macro biphasic calcium phosphate(MBCP) on proliferation and differentiation of human marrow-derived mesenchymal stem cells. Primary stem cells were cultured from bone marrow and 3-4 passaged cells were used. This study tested the proliferative effects by cell counting. Collagen sythensis, alkaline phosphatase activity, expression of osteocalcin and bone sialoprotein by Western blot analysis were evaluated. The cellular proliferation of ASC was not influenced by MBCP. Collagen synthesis of ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The ALP activity in ASC cultured on MBCP significantly increased at 5th and 7th days(p<0.05). The expression of OC and BSP incresaed in ASC cultured on MBCP. These results suggest that MBCP may stimulates the osteoblastic activity of ASC.