Laboratory Animal Research  2016;32(4):200-207

doi:10.5625/lar.2016.32.4.200

Diallyl disulfide attenuates acetaminophen-induced renal injury in rats.

Jin Young SHIN 1 ; Ji Hee HAN ; Je Won KO ; Sung Hyeuk PARK ; Na Rae SHIN ; Tae Yang JUNG ; Hyun A KIM ; Sung Hwan KIM ; In Sik SHIN ; Jong Choon KIM

Affiliations

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Keywords

Acetaminophen; nephrotoxicity; KIM-1; NGAL; diallyl disulfide; protective effect

Country

Republic of Korea

Language

English

Abstract

This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NGAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NGAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NGAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NGAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.