International Journal of Oral Biology  2010;35(3):91-97

Effects of Chitosan on the Differentiation of MDPC-23 Cells.

Ju Hyun PARK 1 ; Do Kyung KIM ; Jong Tae PARK ; Su Young KIM ; Sun Kyoung YU ; Kwang Hee CHO ; Heung Joong KIM

Affiliations

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Keywords

chitosan; odontoblast; differentiation

Country

Republic of Korea

Language

Korean

Abstract

The effects of chitosan upon the experimentally induced differentiation of MDPC-23 cells, derived from mouse dental papilla cells, were investigated by RT-PCR, observations of cell morphology and Alizaline red-S staining. Chitosan was found to significantly increase and accelerate the expression of ALP mRNA but decrease the ColI transcript levels, as compared with the control, in a time-dependent manner during the differentiation of MDPC-23 cells. Chitosan also significantly downregulated ON mRNA expression and accelerated mineralization in differentiating MDPC-23 cells. These results suggest that chitosan facilitates odontoblast differentiation and mineralization and may have potential clinical applications as a dentin regeneration material.