Clinical and Experimental Vaccine Research  2015;4(1):114-118

doi:10.7774/cevr.2015.4.1.114

Antigenic properties and virulence of foot-and-mouth disease virus rescued from full-length cDNA clone of serotype O, typical vaccine strain.

Rae Hyung KIM 1 ; Jia Qi CHU ; Jeong Nam PARK ; Seo Yong LEE ; Yeo Joo LEE ; Mi Kyeong KO ; Ji Hyeon HWANG ; Kwang Nyeong LEE ; Su Mi KIM ; Dongseob TARK ; Young Joon KO ; Hyang Sim LEE ; Min Goo SEO ; Min Eun PARK ; Byounghan KIM ; Jong Hyeon PARK

Affiliations

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Keywords

Foot-and-mouth disease; Vaccine; Molecular cloning

Country

Republic of Korea

Language

English

Abstract

We cloned the full-length cDNA of O Manisa, the virus for vaccinating against foot-and-mouth disease. The antigenic properties of the virus recovered from the cDNA were similar to those of the parental virus. Pathogenesis did not appear in the pigs, dairy goats or suckling mice, but neutralizing antibodies were raised 5-6 days after the virus challenge. The utilization of O Manisa as a safe vaccine strain will increase if recombinant viruses can be manipulated by inserting or removing a marker gene for differential serology or replacing the protective gene from another serotype.