Journal of Periodontal & Implant Science  2010;40(6):265-270

doi:10.5051/jpis.2010.40.6.265

Immunomodulatory effect of canine periodontal ligament stem cells on allogenic and xenogenic peripheral blood mononuclear cells.

Hak Sung KIM 1 ; Kyoung Hwa KIM ; Su Hwan KIM ; Young Sung KIM ; Ki Tae KOO ; Tae Il KIM ; Yang Jo SEOL ; Young KU ; In Chul RHYU ; Chong Pyoung CHUNG ; Yong Moo LEE

Affiliations

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Keywords

Allogenic; Immunomodulation; Periodontal ligaments; Stem cell; Xenogenic

Country

Republic of Korea

Language

English

Abstract

PURPOSE: The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. METHODS: Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established following the addition of phytohemagglutinin. The proliferation of PBMCs was evaluated using the MTS assay. The cell division of PBMCs was analyzed using the CFSE assay. The apoptosis of PBMCs was assessed using the trypan blue uptake method. RESULTS: Periodontal ligament stem cells and bone marrow stem cells inhibited the proliferation of allogenic and xenogenic PBMCs. Both periodontal ligament stem cells and bone marrow stem cells suppressed the cell division of PBMCs despite the existence of a mitogen. No significant differences in the percentages of apoptotic PBMCs were found among the groups. CONCLUSIONS: Canine periodontal ligament stem cells have an immunomodulatory effect on allogenic and xenogenic PBMCs. This effect is not a product of apoptosis of PBMCs but is caused by the inhibition of cell division of PBMCs.