PURPOSE: The aim of this study was to investigate pediatric nurses' perception and knowledge about pediatric hospice and palliative care. METHODS: A cross-sectional descriptive study was conducted with 132 nurses who are stationed in pediatric wards and intensive care units. The participants were requested to fill out a questionnaire comprising 23 items for perception and 20 items for knowledge. Data were collected after obtaining written consent. Data were analyzed using SPSS 21.0. RESULTS: Participants perceived difficulties in pediatric hospice palliative care, especially communication with patients and their families. The differences of perception among participants were observed according to pediatric hospice and palliative care experience, but not general characteristics. Participants' knowledge was 8.83+/-2.64. Nurses who work in a ward showed higher level of knowledge about pediatric hospice/palliative care. CONCLUSION: An education program should be developed and provided to pediatric nurses to improve their perception and knowledge about pediatric hospice/palliative care.