Korean Journal of Pediatric Infectious Diseases  2013;20(3):123-130

Measles Viral Infection in PD-1 Gene Knockout Mice.

Jin Kyong CHUN 1 ; Kyu Yeun KIM ; Ji Ae HUR ; Dong Won KANG ; Ki Hwan KIM ; Dong Soo KIM

Affiliations

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Keywords

Subacute sclerosing panencephalitis; Measles; Programmed death-1

Country

Republic of Korea

Language

Korean

Abstract

PURPOSE: Subacute sclerosing panencephalitis (SSPE) is a neurodegerative disease due to persistent measles virus infection. We investigated the role of programmed death-1 (PD-1) molecule which is related with chronic viral infection in developing SSPE in mouse. METHODS: We adopt the PD-1-/-, PD-1-/+, and wild type BALB/c 3 week old mice to make an animal model of SSPE by injecting measles virus (SSPE strain) intraventricularly. Three months after infusion of virus, the mice were sacrificed and examined if the typical pathologic lesions had been progressed. The sera were collected from each group of mice and the serum level of IL-21 was measured with ELISA kit. RESULTS: The necrotic lesions on white matter and gliosis were found in focal areas in wild type BALB/c. The extent of lesion was smaller in heterotype BALB/c. Scanty lesions were found in PD-1-/- mice. The sera level of IL-21 was not elevated in all three groups. CONCLUSION: Our data suggest that the PD-1 molecule may play a role in persistent viral infection.