Journal of Genetic Medicine  1999;3(1):33-36

Development of Tetracycline-regulated Adenovirus Expression Vector System.

Kyung Hwa SON 1 ; Seung Hoon LEE ; Jong Sik KIM ; Jung Joo CHOI ; Je Ho LEE

Affiliations

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Keywords

Tetracycline-regulated adenovirus; Expression vector

Country

Republic of Korea

Language

English

Abstract

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expresion level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.