Yonsei Medical Journal  1994;35(1):43-48

doi:10.3349/ymj.1994.35.1.43

Serodiagnosis of human sparganosis by a monoclonal antibody-based competition ELISA.

In Sok YEO 1 ; Tai Soon YONG ; Kyung il IM

Affiliations

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Keywords

Sparganosis; Monoclonal antibody; serodiagnosis; competition ELISA

Country

Republic of Korea

Language

English

Abstract

Competition ELISA test using sparganum specific monoclonal antibodies (Mab) was investigated to improve the diagnostic specificity of sparganosis. By cell fusion, one hybridoma clone secreting anti-sparganum specific Mab was selected (Sp-20), which reacted on bands of 32 kDa and 38 kDa. Sp-20 reacted on calcium corpuscles on IFA. By micro-ELISA, 16 of 17 sparganosis cases (95%) were found positive, but 1 of 18 clonorchiasis cases (5%), 4 of 16 cysticercosis cases (25%) and 2 of 16 normal controls (11%) showed false positive reactions. On the other hand, by competition ELISA using a sparganum specific Mab (Sp-20), 16 out of 17 (95%) of sparganosis cases were found positive, but 2 of 18 clonorchiasis cases (10%), 2 of 16 cysticercosis cases (12%), 3 of 16 paragonimiasis cases (18%) and 1 of 16 normal controls (6%) showed false positive reactions.