Environmental Health and Toxicology  2016;31(1):e2016009-

doi:10.5620/eht.e2016009

Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells.

Seung Hyuck BANG 1 ; Dong Jun PARK ; Yang Hoon KIM ; Jiho MIN

Affiliations

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Keywords

Lysosome; Lysosomal membrane proteins; HeLa cell; Melanin; 2-Dimensional electrophoresis

Country

Republic of Korea

Language

English

Abstract

OBJECTIVES: There have been developed to use targeting ability for antimicrobial, anticancerous, gene therapy and cosmetics through analysis of various membrane proteins isolated from cell organelles. METHODS: It was examined about the lysosomal membrane protein extracted from lysosome isolated from HeLa cell treated by 100 ppm melanin for 24 hours in order to find associated with targeting ability to melanin using by 2-dimensional electrophoresis. RESULTS: The result showed 14 up-regulated (1.5-fold) and 13 down-regulated (2.0-fold) spots in relation to melanin exposure. CONCLUSIONS: It has been found that lysosomal membrane proteins are associated with melanin to decolorize and quantity through cellular activation of lysosome.