Annals of Clinical Microbiology  2016;19(1):20-23

doi:10.5145/ACM.2016.19.1.20

An Effective Method of RNA Extraction from Mycobacterium tuberculosis.

Tae Sang OH 1 ; Hee Yoon KANG ; You Sun NAM ; Young Jin KIM ; Eun Kyung YOU ; Min Young LEE ; Sun Young CHO ; Hee Joo LEE

Affiliations

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Keywords

Mycobacterium tuberculosis; RNA extraction; Quantitative reverse transcription polymerase chain reaction

Country

Republic of Korea

Language

English

Abstract

In the RNA-based study, it is important to extract high-quality RNA. However, RNA extraction from Mycobacterium tuberculosis is problematic due to its thick, waxy cell wall rich in mycolic acid, which renders the cells resistant to lysis. Using TRIzol reagent and several powerful bead-beating steps, a high quantity of RNA was obtained.