Korean Journal of Veterinary Research  2016;56(3):189-192

doi:10.14405/kjvr.2016.56.3.189

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency.

Mi Ja PARK 1 ; Seong Joon JOH ; Kang Seuk CHOI ; Aeran KIM ; Min Goo SEO ; Jae Young SONG ; Seon Jong YUN

Affiliations

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Keywords

Massachusetts 41 vaccine; hemagglutination inhibition; infectious bronchitis; potency test

Country

Republic of Korea

Language

English

Abstract

The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.