Korean Journal of Clinical Microbiology  2012;15(3):114-116

doi:10.5145/KJCM.2012.15.3.114

Laboratory Experience in Phenotypic and Molecular Identification of Blastomyces dermatitidis First Isolated in Korea.

Kyung Sun PARK 1 ; Chang Seok KI ; Nam Yong LEE

Affiliations

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Keywords

Blastomyces dermatitidis; Blastomycosis; D1/D2; Internal transcribed spacers (ITS); Molecular identification

Country

Republic of Korea

Language

Korean

Abstract

Blastomycosis, endemic in North America, has been hardly reported in Korea. We describe laboratory experience in phenotypic and molecular identification of Blastomyces dermatitidis first isolated in Korea. The patient was a 45-year-old male with pulmonary blastomycosis mimicking pulmonary tuberculosis. Diagnosis was based on culture and dimorphism combined with DNA target sequencing of internal transcribed spacers (ITS) and D1/D2 regions.