Science of Traditional Chinese Medicine 2025;3(3):270-281

doi:10.1097/st9.0000000000000078

Microbial community mediated by microbial agents improves the quality of Epimedium pubescens Maxim.

Lai KUNYANG 1 ; Wan XIUFU ; Xiao JIANCAI ; Wang HONGYANG ; Shi SHANGXUAN ; Yan BINBIN ; Lyu CHAOGENG ; Zhang CHENGCAI ; Zhang YUFEI ; Yuan FENG ; Zhao ZHE ; Zhu SHOUDONG ; Kang CHUANZHI ; Zhang YAN

Affiliations

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Keywords

Microbial agent; Epimedium pubescens Maxim.; Bioactive components; Rhizosphere microorganisms

Country

China

Language

English

Abstract

Background:Optimizing cultivation techniques for traditional Chinese medicine has become a crucial means to improve the quality of medicinal materials.Microbial agents,as environmentally friendly and efficient plant growth promoters and soil conditioners,have increasingly attracted attention in eco-agriculture research.Objective:Our understanding remains limited regarding how the application of microbial agents,alone or in combination,affects changes in the rhizosphere microbiome and its association with the bioactive components of medicinal materials.Methods:In this study,Epimedium pubescens Maxim.was employed as a model plant to examine the effects of 2 microbial agents(Paenibacillus mucilaginosus and Bacillus subtilis)applied individually and in combination on plant growth and the accumulation of bioactive components.Additionally,this study explored the relationship between the rhizosphere microbiome and plant development.Results:The application of microbial agents increased the yield of E.pubescens leaves by 20.30％to 33.66％and enhanced the total flavonol glycosides content by 11.40％to 29.94％.Meanwhile,microbial treatments reshaped the rhizosphere microbiome,promoted the enrichment of beneficial microorganisms(e.g.,Frankia and Paenibacillus),suppressed phytopathogenic fungi such as Didymella and Scytalidium,and enhanced the stability of the soil microbial co-occurrence network.The partial least squares path model suggested that microbial agents not only directly impact the quality of medicinal herbs but also indirectly alter the accumula-tion of bioactive components by modulating the soil microbiome.Conclusion:These findings deepen our understanding of the relationship between medicinal plant quality and rhizosphere micro-biomes as mediated by microbial agents.They also provide a basis for designing and manipulating synthetic microbial communities to promote sustainable development in eco-agriculture.