Acta Parasitologica et Medica Entomologica Sinica 2000;Ⅶ(1):7-10

doi:10.3969/j.issn.1005-0507.2000.01.002

DETECTION OF PLASMODIUM VIVAX BY NESTED PCR AMPLIFICATION OF DRIED BLOOD SPOTS ON FILTER PAPERS

Chun NIU 1 ; Xinping ZHU ; Lei ZHOU ; Qiang LIU

Affiliations

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Keywords

Plasmodium vivax; nested-PCR; blood filter paper; Chelex

Country

China

Language

Chinese

Abstract

Malaria parasites DNA was eluted from dried blood spot on filter paper by Chelex treatment and amplified by PCR to detect low parasitemia of Plasmodium vivax. Sensitivity and specificity of nested PCR assay were confirmed by amplifying a 121 bp DNA fragment of SSUrRNA gene of Plvivax.Density of about 25 parasites per ul of blood can be detected successfully.None of other three human malaria species(Plasmodium falciparum,Plasmodium malariae and Plasmodium ovale)samples was positive.The ease of collection and transport of filter paper specimens combined with the sensitive and specific detection of P.vivax by nested PCR suggest that this method might be a valuable tool for moleculr epidemiological study of P.vivax.