Objective To explore the pathogenesis of steroid-induced osteonecrosis of the femoral head(SONFH).Methods The SONFH-related deribosomal transcriptome sequencing data set SRP091890 in SRA database was retrieved,and differences in lncRNA and mRNA were obtained by R language analysis.GSE89587,a microarray data set related to SONFH in GEO database,was retrieved,and differential miRNAs were obtained by R language analysis.miRDB,miRTarbase and TargetScan databases were used to predict the target mRNAs of related miRNAs,respectively,to construct a miRNA-mRNA regulatory network.ClusterProfiler package was used for functional enrichment analysis of mRNAs in the above regulatory network.The lncRNA was predicted by Starbase database,and the lncRNA-miRNA-mRNA ceRNA regulatory network was constructed.Results A total of 422 significantly different mRNAs,22 significantly different miRNAs and 416 significantly different lncRNAs were screened by R language analysis.Forty-six mRNAs were identified to be involved in the construction of the SONFH-related miRNA-mRNA regulatory network by cross-mapping the predicted target mRNAs from miRDB,miRTarbase and TargetScan databases and the differentially expressed mRNAs.Through cross-mapping of targeted lncRNAs predicted by Starbase database and lncRNAs with the above differences,3 lncRNAs were determined to be involved in the construction of SONFH-related lncRNA-miRNA regulatory network,and finally the construction of VASH1-AS1-related ceRNA regulatory network.SLC5A6,NQO2 and THAP6 may be indirectly regulated by VASH1-AS1 through hsa-let-7d-5p or hsa-mir-3194-3p.Conclusion VASH1-AS1 may pass from the hsa-let-7d-5p or hsa-mir-3194-3p indirect control SLC5A6,NQO2 and THAP6 and involved in the pathogenesis of SONFH process,and VASH1 AS1,hsa-let-7d-5p and NQO2 may be as a control factor,through regulating network plays a"angiogenesis-bone formation decoupling couplet system",has played a key role in the process of SONFH.