Journal of Sichuan University (Medical Sciences) 2001;32(2):172-174

doi:10.3969/j.issn.1672-173X.2001.02.004

Construction of Recombinant Plasmids Containing Genes of HBsAg and Their Expression in the Eukaryotic Cells

Shan QIN 1 ; Hong TANG ; Liansan ZHAO ; Li LIU ; Yong LIN

Affiliations

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Country

China

Language

Chinese

Abstract

Objective　To construct recombinant plasmids expressing L,M,S and pre-S1-S protein of HBsAg. Methods　Amplifying segments of S, pre-S2-S, pre-S1-pre-S2-S genes of HBV by PCR and amplifying segment of pre-S1-S by overlap extension PCR; inserting the segments into Rc/CMV and pSG5UTPL/Flag plasmids respectively and exploring their expressions by Western-Blot hybridization，identifying the inserting segments by sequencing.Results　The sequences of the inserted segments were the same as the genes of S, pre-S2-S, pre-S1-pre-S2-S and pre-S1-S and the results of Western-Blot hybridization were positive for the aimed proteins. Conclusion　We have gained 8 recombinant plasmids expressing S, M, L and pre-S1-S proteins with high efficacy.