Journal of Pharmaceutical Practice and Service 2025;43(12):610-613

doi:10.12206/j.issn.2097-2024.202412043

Therapeutic drug monitoring and clinical application of fruquintinib

Ming CHEN 1 ; Chenjia WANG 1 ; Yuanjun TANG 1 ; Shuowen WANG 1 ; Guorong FAN 1

Affiliations

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Keywords

fruquintinib; therapeutic drug monitoring; HPLC; individualized medication

Country

China

Language

Chinese

Abstract

Objective To establishing a chromatographic analysis method for therapeutic drug monitoring of fruquintinib by high-performance liquid chromatography （HPLC）. Methods The analysis was conducted using a Welch Ultimate XB-C18 （4.6 mm×150 mm, 5 μm） column with the column temperature at 35℃. The flow rate was 1.0 ml/min and the detection wavelength was at 240 nm. The mobile phase consisted of acetonitrile and 10 mmol/L potassium dihydrogen phosphate （pH 3.15）= 40∶60（V∶V）. The internal standard substance was Apatinib．All plasma samples were extracted with ethyl acetate solution. Results The linearity of Fruquintinib in plasma was good within the range of 15.63-500.00 ng/ml （r=0.999 9）. The recoveries of the method were between 95%-110% with the RSD less than 10%. The extraction rates of the method were all higher than 97%. The stability tests met the relative requirements. Conclusion A HPLC method for the determination of Fruquintinib in human plasma was established. The method had good specialty, and could be used to detect the blood concentrations of Fruquintinib in clinical practice to improve the individualized medication．