Implant-associated infections pose significant challenges for diagnosis and treatment due to the formation of biofilm on device surfaces. Biofilms are complex microbial communities that adhere to surfaces and are encased in a self-produced extracellular polymeric substance (EPS) matrix. Their presence on implants can lead to persistent infections, implant failure, and increased morbidity and mortality. This review explores the role of biofilms in the pathogenesis of implant-associated infections and examines current strategies for their diagnosis and prevention.