Drug Standards of China 2024;25(2):147-153

doi:10.19778/j.chp.2024.02.006

Investigation and determination of relative correction factor of pre-vitamin D

Jieming SHI 1 ; Cheng WANG ; Liwen ZHANG ; Shunling DONG ; Jian LE ; Song YUAN ; Yihong LU ; Dandan WANG ; Wankui XU ; Shufeng ZHENG

Affiliations

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Keywords

vitamin D; pre-vitamin D; relative correction factor; influencing factor; HPLC; external standard method

Country

China

Language

Chinese

Abstract

Objective:To determine the relative correction factor of pre-vitamin D and simplify the calculation method of vitamin D assay.Methods:By studying the calculation method of vitamin D content in drug standards of various countries,HPLC was used to determine the relative correction factor of pre-vitamin D,and the influencing factors of determination were investigated.Results:The relative correction factors of pre-vitamin D at 254 nm and 265nm wavelength were determined by statistical analysis of 7 laboratories in China.Conclusion:Using the pre-vi-tamin D relative correction factor method to calculate the total amount of vitamin D simplified the experimental steps can be simplified by the pre-vitamin D relative correction factor method to calculate the total amount of vitamin D and the random operating errors can be avoided.The method is rapid and accurate,and lay a solid foundation for further improving the standard of vitamin D preparations.