Objective To explore the effect of percutaneous tracheotomy in the clinical application.Methods 40 patients were divided into the observation group and the control group with 20 cases in each group.The observation group underwent the pereutaneous tracheotomy, and the control group received the surgical tracheotomy.The operation time, the incision size, the amount of intra-operative bleeding and postoperative complications were observed between the two groups.Results The operative time in the observation group was significantly shorter than that in the control group (P＜0.01 ), and the incision size smaller than that in the control group (P＜0.01 ).The amount of intra-operative bleeding and the amount of bleeding 24 hrs after the operation were small, and the postoperative complications reduced.Conclusions Compared to the surgical tracheotomy (ST), the pereutaneous dilational tracheotomy (PDT) has advantages of shorter surgery time, smaller incision size, less bleeding and fewer complications.So PDT is a tracheotomy method that is easy to be conducted near the bed, safe and practical, thereby being worthy of clinical application.