Laboratory Animal Research  2010;26(2):219-221

doi:10.5625/lar.2010.26.2.219

Genetic Polymorphism of Jeju Horses by Microsatellite DNA Markers in Korea.

Jung Eun LEE 1 ; Jae Hoon SHIN ; Young Min YUN ; Kyung Kap LEE ; Hang LEE ; Oh Kyung KWEON ; Yeo Sung YUN ; Jun Gyo SUH ; Nam Shik SHIN ; Je Kyung SEONG

Affiliations

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Keywords

Jeju horses; genetic polymorphism; microsatellite DNA

Country

Republic of Korea

Language

English

Abstract

We determined the genetic diversity and distance between Jeju and Thoroughbred horses by genotyping for 20 microsatellite loci consisting of (TG)n repetitive sequence. The expected heterozygosity ranged from 0.1 to 0.789 in the Jeju horses and from 0.505 to 0.824 in the Thoroughbred horses. Polymorphic information content (PIC) values ranged from 0.09 to 0.709 in the Jeju horses and 0.365 to 0.730 in Thoroughbred horses. There were no significant differences in heterozygosity and PIC values between Jeju and Thoroughbred horsesHowever, LEX035 was estimated relatively high heterozygosity (0.789) and PIC value 0.709) in Jeju horses and LEX050 was respectively 0.824, and 0.730 in the Thoroughbred horses. We may conclude that the genetic differentiation was low between Jeju and Thoroughbred horses. LEX 050, LEX055, LEX059 and LEX 063 could be used as geneticmarkers for differentiating Jeju from Thoroughbred horses.