OBJECTIVE To investigate proliferative activities for peritoneal macrophages and splenic lymphocytes in mice and anti-oxidative potencyinvitro ofn-butanol extract ofRadixisatidis.METHODS 12 components were extracted and sep-arated from Radix isatidis by n-butanol.Each component of total n-butanol extracts was detected and analyzed by HPLC. The proliferative activities of each component for peritoneal macrophages and splenic lymphocytes were detected by MTT as-say.Anti-oxidative activities were evaluated by DPPH and ABTS radical scavenging and FRAP method.RESULTS No.2,6, 7 and 11 components showed the most potent proliferation on immune cells,with the highest proliferation index>3.No.2 and 6 components displayed the strongest antioxidant activities,with IC50 values on DPPH of 0.391,0.164 mg/mL,ABTS of 0. 164,0.338 mg/mL and Fe3+ reducibility values of 1.441,0.812 mmol/g equivalent weight of FeSO4 ,respectively.CONCLU-SION The n-butanol extract of Radix isatidis exhibit promoting proliferation for peritoneal macrophages and splenic lympho-cytes and pontent antioxidant activities.Further investigation of the chemical structure will help to clarify the material basis for Radix isatidis antiviral effects.